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KMID : 0613820030130050705
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Journal of Life Science
2003 Volume.13 No. 5 p.705 ~ p.711
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Optimization of culture condition for the gellan production by Pseudomonas elodea ATCC 31461
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Lim Sung-Mi
Lee Jin-Woo
Kim Sung-Koo
Jian Rong Wu
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Abstract
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The gellan was produced by Pseudomonas elodea under aerobic condition. In this study, the effects of inoculum size, carbon sources and concentration, nitrogen source, and C/N ratio on the cell growth and the production of gellan were evaluated. The maximum growth of P. elodea and gellan production was obtained at 5% (v/v) of inoculum size and glucose showed best results among 9 carbon sources tested. The maximum specific yield of 2.22 and productivity of 0.03 g/ℓ/h were obtained at 1.0% (w/v) of glucose. The maximum gellan production was obtained at medium without ammonium nitrate. This indicates that nitrogen limitation is essential for the production of gellan. The highest cell and gellan production were obtained at 20 of C/N ratio.
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KEYWORD
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fermentation, gellan, optimum culture condition, Pseudomonas elodea
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